GHK-Cu / KPV Blend Testing & Verification
Independent, third-party analysis of GHK-Cu / KPV blend vials by RP-HPLC-UV — two small, early-eluting peptides in one vial, resolved and reported separately on one certificate, with a prep that keeps the copper complex intact.
What GHK-Cu / KPV is
GHK-Cu / KPV is a two-active vial most commonly labelled as a 50/10 mg split. Both actives are tripeptides — GHK-Cu a copper-complexed glycine-histidine-lysine, KPV the lysine-proline-valine fragment of α-MSH — which makes this the blend where the two peaks sit closest to the solvent front and closest to each other, and where the method has to resolve them without the acidic diluent that would strip GHK's copper. Each is quantified against its own certified reference standard; a single purity figure for the vial would be meaningless. Our certificate leads with the name on your label and prints the two actives beneath it, each with its own results row.
Why get your GHK-Cu / KPV independently tested
- Two early peaks that must be told apart: both actives are small and polar. Identity by retention-time and UV-spectral match against separate GHK and KPV standards confirms both are present and which peak is which.
- Copper-complex integrity: acid strips the copper from GHK-Cu, so a blend prepped carelessly measures a damaged sample. Our prep for copper peptides is acid-free, and appearance — a blue solution when the complex is intact — is reported on the certificate.
- The 10 mg component is the one to skip: KPV is cheap and small, and a fill that leaves it out still looks like 50 mg of something. Per-active identity settles whether it is there.
- Content on two tiny molecules: at roughly 340 Da (KPV) and ~400 Da (GHK), small mass errors are large fractions of each claim. Per-active net-peptide content reports each measured against its declared share.
What we measure on GHK-Cu / KPV
A GHK-Cu / KPV certificate reports, for EACH of the two actives, by reversed-phase HPLC with diode-array detection at 214 nm: identity (retention-time and UV-spectral match against that compound's own certified reference standard), chromatographic purity as area percent, content in milligrams per vial, and retention time — one vial, one certificate, one verification code, two fully reported compounds. The copper ion dissociates on the column, so GHK-Cu's result is stated for the GHK peptide. Appearance (blue when the copper complex is intact) and optional pH are reported at the vial level.
Every result is issued as a certificate of analysis you — or anyone you forward it to — can verify cryptographically. Reporting identity, purity, and milligram content is the point: a vial can be pure and still underfilled, so we report all three. More on purity vs. net-peptide content and how to read a peptide COA.
Our scope — stated plainly
Testing is RP-HPLC-UV against certified reference standards for each declared active — not mass spectrometry. The certificate reports the actives declared on your label; it does not de-novo identify unlabelled compounds, and it does not quantify free copper. KMD Analytical is an independent laboratory; we do not perform sterility, endotoxin, heavy-metal, or residual-solvent testing.
KMD is an independent RP-HPLC-UV lab. We do not run LC-MS / mass-spec, sterility, endotoxin, or heavy-metal testing. If your job needs a capability we don't have, we'll say so.
GHK-Cu / KPV testing — FAQ
Can two tripeptides be resolved from each other?
Yes. GHK and KPV differ in sequence and charge and separate under the conditions we use for polar peptides; each is identified against its own reference standard and quantified on its own calibration. The method is chosen so both peaks are clear of the solvent front.
Why does the prep matter for this blend?
Acid strips the copper from GHK-Cu. We reconstitute and dilute copper-peptide blends without acid so the vial is measured as it was made, not as our diluent changed it.
My split isn't 50/10 — can you still test it?
Yes. The 50/10 split is only the most common label; the blend preset pre-fills it when you order and you edit it to match your vial. Each active is verified against whatever your label declares.
Do I get one certificate or two?
One. A blend vial produces a single certificate with one verification code, on which each declared active has its own identity, purity, content, and retention-time row.
What's in this blend, and related tests
GHK-Cu / KPV is a blend, so it prices per active — the tier rate ($150/vial, $125 at 5+, $100 at 10+) plus 50% for each additional active, since every compound in the vial is resolved and reported separately. Rush results in 4 business days. Start an order or see the full rate card.