Epithalon (Epitalon) Testing & Verification
Independent, third-party analysis of your Epithalon (Epitalon) sample by reversed-phase HPLC with UV detection — confirming what is actually in the vial before you rely on the label.
What Epithalon is
Epithalon (also spelled Epitalon) is a synthetic linear tetrapeptide — a four-residue chain with the sequence Ala-Glu-Asp-Gly (AEDG) and a molecular weight near 390 g/mol. It is a short, unmodified peptide with no disulfide bonds and no metal complex, and notably it contains no aromatic amino acids, so it is detected chromatographically by the peptide-bond absorbance at 214 nm rather than at 280 nm. It is manufactured by solid-phase peptide synthesis and supplied as a lyophilized powder on the research-chemical market rather than as an approved drug, which means the identity, purity, and content printed on a vial carry no regulatory oversight.
Why get your Epithalon independently tested
- Short sequence with no visual tells: a lyophilized white powder labeled 'Epithalon' looks identical by eye to a cheaper peptide, a related analog, or inert filler, so identity has to be confirmed instrumentally against a certified reference standard rather than taken on trust.
- Lyophilized vial mass is not peptide mass: freeze-dried powder also contains counter-ions (such as acetate or TFA salts), residual water, and any bulking agent, so a vial labeled by milligrams can hold noticeably less actual peptide — the net-peptide assay reports milligrams of peptide per vial, not total powder weight.
- Acidic-residue instability: the glutamate and aspartate residues are positions associated with isomerization and degradation during storage and shipping, so the purity of the specific vial you received can differ from any batch figure a supplier quotes.
- Synthesis-related impurities: solid-phase synthesis of a short peptide routinely leaves truncation and deletion-sequence impurities plus process-related by-products; chromatographic purity as area percent quantifies those related substances instead of assuming a seller's stated figure.
What we measure on Epithalon
Each Epithalon sample is analyzed by RP-HPLC with UV/diode-array detection at 214 nm against a certified reference standard — because the peptide has no aromatic chromophore, detection relies on the amide-bond absorbance at 214 nm. The certificate reports exactly three results: identity (retention-time plus UV-spectral match to the reference), chromatographic purity as area percent, and net-peptide assay as milligrams of peptide per vial.
Every result is issued as a certificate of analysis you — or anyone you forward it to — can verify cryptographically. Reporting identity, purity, and milligram content is the point: a vial can be pure and still underfilled, so we report all three. More on purity vs. net-peptide content and how to read a peptide COA.
Our scope — stated plainly
Testing is by RP-HPLC-UV against a certified reference standard; it confirms identity, purity, and content but does not include mass spectrometry, sterility, or endotoxin testing. Epithalon reference standards are commercially available, so identity testing does not usually require a sourcing delay. KMD Analytical is an independent laboratory.
KMD is an independent RP-HPLC-UV lab. We do not run LC-MS / mass-spec, sterility, endotoxin, or heavy-metal testing. If your job needs a capability we don't have, we'll say so.
Epithalon testing — FAQ
How do you confirm a sample is actually Epithalon?
Identity is assigned by comparing your sample to a certified Epithalon reference standard run under the same RP-HPLC-UV conditions, matching both retention time and the UV spectrum. Because Epithalon has no aromatic residues, detection uses the peptide-bond absorbance at 214 nm. Note this is a chromatographic and spectral confirmation, not a mass-spectrometry (molecular-weight) confirmation.
What does 'net peptide content' mean for an Epithalon vial?
It is the milligrams of actual Epithalon peptide in the vial, which is not the same as the total weight of the lyophilized powder. That powder also includes salt counter-ions, residual moisture, and any bulking agent, so the labeled milligram figure and the true peptide content can differ. Our assay reports the peptide mass per vial specifically.
What exactly do I get on the certificate?
Three results: identity (match to a certified reference standard), chromatographic purity as area percent, and net-peptide assay in milligrams per vial. Mail in your sample and we analyze it as received.
Other peptides we test
Retatrutide · Cagrilintide · Tesamorelin · BPC-157 · see the full panel.