Mito Blend Testing & Verification (NAD+ · MOTS-c · 5-Amino-1MQ)
Independent, third-party analysis of Mito Blend vials by HPLC-UV — three very different molecules in one vial, each resolved and quantified on the method and wavelength that suits it, all reported on one certificate.
What Mito Blend is
Mito Blend is a trade name for one vial carrying NAD+, MOTS-c, and 5-Amino-1MQ together, most commonly labelled as a 100/10/10 mg split. It is the most chemically mixed blend we run: NAD+ is a dinucleotide, not a peptide, read at 260 nm and unstable in solution; MOTS-c is a 16-residue peptide read at the usual peptide wavelength; and 5-Amino-1MQ is a small quaternary-ammonium molecule that needs its own method and, because no certified standard exists for it from our usual sources, is identified against characterised reference material rather than a certified one. A single 'purity' number for that vial would be meaningless; each active has to be resolved and quantified on terms that suit its chemistry. Our certificate leads with the name on your label (Mito Blend) and prints the actual actives beneath it, each with its own results row and its own stated method.
Why get your Mito Blend independently tested
- Split verification across unlike chemistries: a Mito Blend label declares three actives with three different analytical needs. Each gets its own identity, purity, and content line, so a short or missing component cannot hide inside a healthy-looking total — and the dominant NAD+ mass cannot mask a missing MOTS-c.
- NAD+ stability: NAD+ degrades in solution, so a vial that was reconstituted and refrozen, or made up in solution and shipped, may already be short. Content against a fresh NAD+ standard reports what survived.
- The small-molecule component: 5-Amino-1MQ is not a peptide and does not respond on the peptide method. Testing it on its own conditions, against characterised reference material, is the only way its line on the certificate means anything.
- Missing-component risk: the expensive actives in this recipe are the two small ones by mass. Per-component identity against each active's own reference settles whether all three compounds are actually present.
What we measure on Mito Blend
A Mito Blend certificate reports, for EACH of the three actives, by HPLC with diode-array detection: identity (retention-time and UV-spectral match against that active's own reference standard or, for 5-Amino-1MQ, characterised reference material), chromatographic purity as area percent, content in milligrams per vial, and retention time — with NAD+ read at 260 nm, MOTS-c at 214 nm, and 5-Amino-1MQ on its own method. One vial, one certificate, one verification code, three fully reported actives, each with its method stated. Appearance and optional pH are reported at the vial level.
Every result is issued as a certificate of analysis you — or anyone you forward it to — can verify cryptographically. Reporting identity, purity, and milligram content is the point: a vial can be pure and still underfilled, so we report all three. More on purity vs. net-peptide content and how to read a peptide COA.
Our scope — stated plainly
Testing is HPLC-UV against a reference standard for each declared active; 5-Amino-1MQ has no certified reference standard from our usual sources and is identified against characterised reference material, which the certificate states. This is not mass spectrometry. The certificate reports the actives declared on your label; it does not de-novo identify unlabelled compounds. KMD Analytical is an independent laboratory; we do not perform sterility, endotoxin, heavy-metal, or residual-solvent testing.
KMD is an independent RP-HPLC-UV lab. We do not run LC-MS / mass-spec, sterility, endotoxin, or heavy-metal testing. If your job needs a capability we don't have, we'll say so.
Mito Blend testing — FAQ
How can one vial be tested when the three actives are so different?
By not pretending they are alike. Each active is resolved and quantified under the conditions that suit it — NAD+ at 260 nm, MOTS-c at the peptide wavelength, 5-Amino-1MQ on its own small-molecule method — and the certificate states the method beside each result. It is more bench work than a single-compound vial, which is why blends price per active.
Why does the certificate say 'characterised reference material' for 5-Amino-1MQ?
Because that is the truth. No certified reference standard for 5-Amino-1MQ is available from our usual suppliers, so identity is judged by retention time and UV spectrum against reference material we have characterised in-house. We say 'characterised', never 'certified', when that is what was used.
My Mito Blend is already in solution — can you test it?
Yes, with a caveat: NAD+ degrades in solution, so content reflects what is in the vial when we test it, not what was put in. Tell us the form on the order form; lyophilised vials give the cleanest answer.
Do I get one certificate or three?
One. A blend vial produces a single certificate with one verification code, on which each declared active has its own identity, purity, content, retention-time, and method row. One physical vial never becomes multiple certificates.
What's in this blend, and related tests
NAD+ · MOTS-c · 5-Amino-1MQ · Retatrutide · Cagrilintide · see the full panel.
Mito Blend is a blend, so it prices per active — the tier rate ($150/vial, $125 at 5+, $100 at 10+) plus 50% for each additional active, since every compound in the vial is resolved and reported separately. Rush results in 4 business days. Start an order or see the full rate card.